GSM4160370: T4.RNA-seq.NBL; Trichinella pseudospiralis; RNA-Seq - SRA

SRX7141124: GSM4160370: T4.RNA-seq.NBL; Trichinella pseudospiralis; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2500) run: 33.9M spots, 10.2G bases, 3.1Gb downloads

Submitted by: NCBI (GEO)

Study: 12 transcriptomes of three life stages using RNA-Seq

PRJNA589551 • SRP229961 • All experiments • All runs

show Abstracthide Abstract

As DNA methylation can modulate gene expression, we then focused our analysis on changes of methylation at the host taxonomic level, that is between encapsulated and non-encapsulated clade, and within non-encapsulated clade, to reveal epigenetic regulation on transcriptomes from Ad and ML stages. By comparing DNA methylation patterns between different host classes of species, we found a fraction of parasitism-related genes under epigenetic regulation, such as G-protein-coupled receptor, DNaseII and ligand-gated chloride channel. Overall design: We report 12 transcriptomes of three life stages using RNA-Seq

Sample: T4.RNA-seq.NBL

SAMN13283358 • SRS5649265 • All experiments • All runs

Organism: Trichinella pseudospiralis

Library:

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Total RNA of T. spiralis (Ad, NBL and ML) was purified using Trizol reagent (Invitrogen, CA, USA) according to the manufacturer's instructions. To degrade any possible DNA contamination, the total RNA samples were dissolved in diethylpyrocarbonate (DEPC)-treated water and treated with DNaseI. NanoDrop 2000 (Thermo Scientific CA, USA) was then used to assess the quantity and quality of the total mRNA enriched using oligo (dT) magnetic beads. Paired-end sequencing libraries with insert size of 300 bp were conducted on the HiSeq 2,500 platform following the Illumina's protocols.

Experiment attributes:

GEO Accession: GSM4160370

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 33.9M spots, 10.2G bases, 3.1Gb

Run	# of Spots	# of Bases	Size	Published
SRR10446413	33,911,297	10.2G	3.1Gb	2020-06-01

ID:: 9386067

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Sequence Read Archive

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